Summary: The second session focuses on the scientific method, experiment design, and essential laboratory techniques. Participants will learn about literature research, molecular biology tools, and best practices for conducting and documenting experiments.

Cost for Attendance: $33 (payment links @ door or in advance to @elliot-roth // @thatmre)

Part II Topics & Agenda

• Scientific Method & Experimental Design (30 min)

  • Subtopics: Defining a problem, designing experiments, establishing controls, laboratory notebooks

• Literature Research (15 min)

  • Subtopics: Effective literature searching, PubMed, Google Scholar

• Tools for Molecular Biology (30 min)

  • Subtopics: Online databases, DNA synthesis, metabolic engineering tools

• Wet Lab Methods (90 min)

  • Subtopics:

    • Biosafety and sterile techniques

    • Culturing microorganisms

    • DNA extraction and sequence analysis

    • DNA cloning/assembly: PCR, restriction-ligation, Gibson assembly, gel electrophoresis, cell transformation

• Scientific Figures (15 min)

  • Subtopics: Creating clear and accurate scientific images

Slides: https://drive.google.com/drive/u/0/folders/15yWl_fZ7AUSJJvcyxPrMsO9WSU_gJv60

• Good scientific image practice workflows and guides:

  • 📝 Effective image visualization for publications –  workflow using open access tools and concepts | F1000Research

  • 📝 Community-developed checklists for publishing images and image analyses | Nature Methods

  • 📝 3 ways to make your scientific images accurate, informative and accessible | News | Nature Index

• Literature Research

• 📝 Google Scholar Keyword Search: Advanced Searching - Google Scholar - Research Guides at Southeast Missouri State University

📝 Pubmed Keyword Search: Searching in PubMed - PubMed - Research Guides at Harvard Library